# User:Allison K. Alix/Notebook/Thesis Research/2013/01/29

(Difference between revisions)
 Revision as of 20:04, 4 February 2013 (view source) (→Procedures)← Previous diff Revision as of 20:08, 4 February 2013 (view source) (→Procedures)Next diff → Line 13: Line 13: ==Procedures== ==Procedures== + + '''ZnPPIX:''' 5μM ZnPPIX already prepared 5μM ZnPPIX already prepared - ''To prepare 1 μM 4 telomeric repeat DNA:'' + 1. Prepare 1 μM 4 telomeric repeat DNA: 265.6μM (x) = 1μM (100μL) 265.6μM (x) = 1μM (100μL) Line 22: Line 24: x = '''2.656 μL''' in 97.344 μL H2O x = '''2.656 μL''' in 97.344 μL H2O - ''To prepare 1 μM 8 telomeric repeat DNA:'' + 2. Prepare 1 μM 8 telomeric repeat DNA 142.8μM (x) = 1μM (100μL) 142.8μM (x) = 1μM (100μL) x = '''1.428 μL''' in 98.572 μL H2O x = '''1.428 μL''' in 98.572 μL H2O + + '''ThT''' + + 1. prepare 5mM tris-HCl buffer solution + + 121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL + + Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 + + 2. +

## Revision as of 20:08, 4 February 2013

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## Objectives

-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1

-Hybridize ThT and DNA strands

## Procedures

ZnPPIX:

1. Prepare 1 μM 4 telomeric repeat DNA:

265.6μM (x) = 1μM (100μL)

x = 2.656 μL in 97.344 μL H2O

2. Prepare 1 μM 8 telomeric repeat DNA

142.8μM (x) = 1μM (100μL)

x = 1.428 μL in 98.572 μL H2O

ThT

1. prepare 5mM tris-HCl buffer solution

```  121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL
```
```  Add 1 N HCl (~8 drops) to obtain pH ~ 7.25
```

2.