User:Allison K. Alix/Notebook/Thesis Research/2013/01/29

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(Procedures)
(Procedures)
Line 16: Line 16:
5μM ZnPPIX already prepared
5μM ZnPPIX already prepared
-
'''To prepare 1 μM 4 telomeric repeat DNA:'''
+
''To prepare 1 μM 4 telomeric repeat DNA:''
265.6μM (x) = 1μM (100μL)
265.6μM (x) = 1μM (100μL)
-
x = 2.656 μL in 97.344 μL H<sub>2</sub>O
+
x = '''2.656 μL''' in 97.344 μL H<sub>2</sub>O
-
'''To prepare 1 μM 8 telomeric repeat DNA:'''
+
''To prepare 1 μM 8 telomeric repeat DNA:''
142.8μM (x) = 1μM (100μL)
142.8μM (x) = 1μM (100μL)
-
x = 1.428 μL in 98.572 μL H<sub>2</sub>O
+
x = '''1.428 μL''' in 98.572 μL H<sub>2</sub>O
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Revision as of 19:04, 4 February 2013

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Objectives

-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1

-Hybridize ThT and DNA strands

Procedures

5μM ZnPPIX already prepared

To prepare 1 μM 4 telomeric repeat DNA:

265.6μM (x) = 1μM (100μL)

x = 2.656 μL in 97.344 μL H2O

To prepare 1 μM 8 telomeric repeat DNA:

142.8μM (x) = 1μM (100μL)

x = 1.428 μL in 98.572 μL H2O


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