User:Allison K. Alix/Notebook/Thesis Research/2013/01/15 - Revision history

Allison K. Alix: /* Data */

Allison K. Alix — Tue, 15 Jan 2013 20:14:59 GMT

Data

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	==Data==		==Data==
		+
	[[Image:FluorSpecAll.jpg]]		[[Image:FluorSpecAll.jpg]]
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	__NOTOC__		__NOTOC__

Allison K. Alix: /* Data */

Allison K. Alix — Tue, 15 Jan 2013 20:13:27 GMT

Data

←Older revision		Revision as of 20:13, 15 January 2013
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	==Data==		==Data==
-		+	[[Image:FluorSpecAll.jpg]]
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	__NOTOC__		__NOTOC__

Allison K. Alix: /* Procedures */

Allison K. Alix — Tue, 15 Jan 2013 19:59:30 GMT

Procedures

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	Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.		Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.
		+
		+	==Data==

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Allison K. Alix: /* Procedures */

Allison K. Alix — Tue, 15 Jan 2013 19:59:03 GMT

Procedures

←Older revision		Revision as of 19:59, 15 January 2013
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	==Procedures==		==Procedures==

-	* Fluorescence Spectra for the following samples ~~were obtained~~ (excited at 515nm)	+	Obtain fluorescence spectra for the following samples:(excited at 515nm)

-	* concentrated AuNP - (3x10^-9 M)	+	* concentrated AuNP (3x10^-9 M)
	* 1:6 diluted AuNP (6.49 x10^-9 M)		* 1:6 diluted AuNP (6.49 x10^-9 M)
-	* 1:6 ~~dilution~~ with thiol primer attached. (10μL diluted to 100μL with 90μL water)	+	* 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
-	* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\	+	* Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
	* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)		* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
		+
		+	Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Allison K. Alix: /* Re-running Fluorescence Measurements */

Allison K. Alix — Tue, 15 Jan 2013 19:54:25 GMT

Re-running Fluorescence Measurements

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	\| colspan="2"\|		\| colspan="2"\|
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-	==~~Re-running Fluorescence Measurements~~==	+	==Procedures==

	* Fluorescence Spectra for the following samples were obtained (excited at 515nm)		* Fluorescence Spectra for the following samples were obtained (excited at 515nm)

Allison K. Alix at 19:54, 15 January 2013

Allison K. Alix — Tue, 15 Jan 2013 19:54:05 GMT

←Older revision		Revision as of 19:54, 15 January 2013
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	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> Fluorescence Measurements</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-

Allison K. Alix: /* Re-running Fluorescence Measurements */

Allison K. Alix — Tue, 15 Jan 2013 19:52:00 GMT

Re-running Fluorescence Measurements

←Older revision		Revision as of 19:52, 15 January 2013
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	==Re-running Fluorescence Measurements==		==Re-running Fluorescence Measurements==
		+
	* Fluorescence Spectra for the following samples were obtained (excited at 515nm)		* Fluorescence Spectra for the following samples were obtained (excited at 515nm)

-	* concentrated AuNP - (3x10^-9 M)~~excited at 515nm between 530 and 680~~	+	* concentrated AuNP - (3x10^-9 M)
-	* 1:6 diluted AuNP (6.49 x10^-9 M) ~~excited at 515nm between 530nm and 680nm~~	+	* 1:6 diluted AuNP (6.49 x10^-9 M)
	* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)		* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)
	* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\		* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\

Allison K. Alix: /* Re-running Fluorescence Measurements */

Allison K. Alix — Tue, 15 Jan 2013 17:24:45 GMT

Re-running Fluorescence Measurements

←Older revision		Revision as of 17:24, 15 January 2013
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	==Re-running Fluorescence Measurements==		==Re-running Fluorescence Measurements==
-	* Fluorescence Spectra for the following samples were obtained:	+	* Fluorescence Spectra for the following samples were obtained (excited at 515nm)

-	*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680	+	* concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680
	* 1:6 diluted AuNP (6.49 x10^-9 M) excited at 515nm between 530nm and 680nm		* 1:6 diluted AuNP (6.49 x10^-9 M) excited at 515nm between 530nm and 680nm
-	* 1:6 dilution with thiol primer attached. (~~10microL~~ diluted to ~~100microL~~ with ~~90 microL~~ water)	+	* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)
		+	* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
		+	* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)

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Allison K. Alix: /* Re-running Fluorescence Measurements */

Allison K. Alix — Tue, 15 Jan 2013 17:18:01 GMT

Re-running Fluorescence Measurements

←Older revision		Revision as of 17:18, 15 January 2013
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	*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680		*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680
	* 1:6 diluted AuNP (6.49 x10^-9 M) excited at 515nm between 530nm and 680nm		* 1:6 diluted AuNP (6.49 x10^-9 M) excited at 515nm between 530nm and 680nm
-		+	* 1:6 dilution with thiol primer attached. (10microL diluted to 100microL with 90 microL water)

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Allison K. Alix: /* Re-running Fluorescence Measurements */

Allison K. Alix — Tue, 15 Jan 2013 17:14:40 GMT

Re-running Fluorescence Measurements

←Older revision		Revision as of 17:14, 15 January 2013
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	*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680		*concentrated AuNP - (3x10^-9 M)excited at 515nm between 530 and 680
		+	* 1:6 diluted AuNP (6.49 x10^-9 M) excited at 515nm between 530nm and 680nm