User:Allison K. Alix/Notebook/Thesis Research/2013/01/15: Difference between revisions
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==Procedures== | ==Procedures== | ||
Obtain fluorescence spectra for the following samples:(excited at 515nm) | |||
* concentrated AuNP | * concentrated AuNP (3x10^-9 M) | ||
* 1:6 diluted AuNP (6.49 x10^-9 M) | * 1:6 diluted AuNP (6.49 x10^-9 M) | ||
* 1:6 | * 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water) | ||
* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\ | * Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\ | ||
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O) | * Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O) | ||
Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer. | |||
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Revision as of 12:59, 15 January 2013
 Fluorescence Measurements
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ProceduresObtain fluorescence spectra for the following samples:(excited at 515nm)
Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer. | |
