User:Allison K. Alix/Notebook/Thesis Research/2013/01/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Procedures== | ||
Obtain fluorescence spectra for the following samples:(excited at 515nm) | |||
* concentrated AuNP | * concentrated AuNP (3x10^-9 M) | ||
* 1:6 diluted AuNP (6.49 x10^-9 M) | * 1:6 diluted AuNP (6.49 x10^-9 M) | ||
* 1:6 | * 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water) | ||
* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\ | * Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\ | ||
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O) | * Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O) | ||
Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer. | |||
==Data== | |||
[[Image:FluorSpecAll.jpg]] | |||
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Revision as of 13:14, 15 January 2013
Fluorescence Measurements | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ProceduresObtain fluorescence spectra for the following samples:(excited at 515nm)
Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer. Data |