User:Allison K. Alix/Notebook/Thesis Research/2013/01/15

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(Re-running Fluorescence Measurements)
Current revision (16:14, 15 January 2013) (view source)
(Data)
 
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==Procedures==
==Procedures==
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* Fluorescence Spectra for the following samples were obtained (excited at 515nm)
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Obtain fluorescence spectra for the following samples:(excited at 515nm)
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* concentrated AuNP - (3x10^-9 M)
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* concentrated AuNP (3x10^-9 M)
* 1:6 diluted AuNP (6.49 x10^-9 M)  
* 1:6 diluted AuNP (6.49 x10^-9 M)  
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* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)
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* 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
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* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
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* Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
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 +
Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.
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==Data==
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[[Image:FluorSpecAll.jpg]]
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Fluorescence Measurements Main project page
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Procedures

Obtain fluorescence spectra for the following samples:(excited at 515nm)

  • concentrated AuNP (3x10^-9 M)
  • 1:6 diluted AuNP (6.49 x10^-9 M)
  • 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
  • Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H2O)\
  • Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H2O)

Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer.

Data

Image:FluorSpecAll.jpg


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