User:Allison K. Alix/Notebook/Fluorescence Detection of Telomerase Activity/2012/11/15 - Revision history

Allison K. Alix: /* Procedure */

2012-11-15T20:26:06Z

Procedure

←Older revision		Revision as of 20:26, 15 November 2012
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	==Procedure==		==Procedure==
		+
		+	Preparation of Mixtures:
		+
		+	1) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand, 25μL Molecular Beacon
		+	b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand, 25μL Molecular Beacon
		+
		+	2) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL Molecular Beacon
		+	b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL Molecular Beacon
		+
		+	3) 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 10μL 5μM Zinc Protoporphyrin IX
		+
		+	4) 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 10μL 5μM Zinc Protoporphyrin IX
		+
		+	5) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand
		+	b. b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand

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Allison K. Alix: /* Objectives */

2012-11-15T20:16:07Z

Objectives

←Older revision		Revision as of 20:16, 15 November 2012
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	-take fluorescence measurements of several mixtures all of which contain cancer cell solution		-take fluorescence measurements of several mixtures all of which contain cancer cell solution

		+
		+	==Procedure==

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Allison K. Alix: /* Entry title */

2012-11-15T20:15:45Z

Entry title

←Older revision		Revision as of 20:15, 15 November 2012
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-	==~~Entry title~~==	+	==Objectives==
-	* Insert content here...	+
		+	-take fluorescence measurements of several mixtures all of which contain cancer cell solution

Allison K. Alix: Autocreate 2012/11/15 Entry for User:Allison_K._Alix/Notebook/Fluorescence_Detection_of_Telomerase_Activity

2012-11-15T20:14:09Z

Autocreate 2012/11/15 Entry for User:Allison_K._Alix/Notebook/Fluorescence_Detection_of_Telomerase_Activity

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==Entry title==
* Insert content here...


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