User:Allison K. Alix/Notebook/CHEM-581/2013/04/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objectives== | ==Objectives== | ||
* Sonicate hydrogels containing dye using ultra sonic liquid processing and filter | * Sonicate hydrogels containing dye using ultra sonic liquid processing and filter (2x) | ||
* Mix sonicated hydrogels containing no dye with dye and indicators to monitor absorbance | |||
==Procedures== | ==Procedures== | ||
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5) Take absorbance of remaining filtrant | 5) Take absorbance of remaining filtrant | ||
6) Redisperse hydrogels on filter paper back into phosphate buffer and observe under microscope | 6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope | ||
Part 2: Mixing ultrasonicated hydrogels | |||
The following samples were prepared: | |||
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==Data== | ==Data== |
Latest revision as of 22:37, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
Objectives
ProceduresPart 1: Sonication of Hydrogels 1) Measure ~7.5mL of solution C 2) Sonicate for ~5 minutes at 15 watts 3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast) 4) Filter the filtrant once more through a nylon filter. 5) Take absorbance of remaining filtrant 6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope Part 2: Mixing ultrasonicated hydrogels The following samples were prepared: Data
NotesThis area is for any observations or conclusions that you would like to note.
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