User:Allison K. Alix/Notebook/CHEM-581/2013/04/12

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Current revision (14:39, 12 April 2013) (view source)
(Procedures)
 
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==Objectives==
==Objectives==
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* Sonicate hydrogels containing dye using ultra sonic liquid processing and filter
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* Sonicate hydrogels containing dye using ultra sonic liquid processing and filter (2x)
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* Mix sonicated hydrogels containing no dye with dye and indicators to monitor absorbance
==Procedures==
==Procedures==
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Part 1: Sonication of Hydrogels
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'''Part 1: Sonication of Hydrogels'''
1) Measure ~7.5mL of solution C
1) Measure ~7.5mL of solution C
2) Sonicate for ~5 minutes at 15 watts
2) Sonicate for ~5 minutes at 15 watts
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 +
3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast)
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4) Filter the filtrant once more through a nylon filter.
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5) Take absorbance of remaining filtrant
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6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope
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Part 2: Mixing ultrasonicated hydrogels
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The following samples were prepared:
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*
==Data==
==Data==

Current revision

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Objectives

  • Sonicate hydrogels containing dye using ultra sonic liquid processing and filter (2x)
  • Mix sonicated hydrogels containing no dye with dye and indicators to monitor absorbance

Procedures

Part 1: Sonication of Hydrogels

1) Measure ~7.5mL of solution C

2) Sonicate for ~5 minutes at 15 watts

3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast)

4) Filter the filtrant once more through a nylon filter.

5) Take absorbance of remaining filtrant

6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope

Part 2: Mixing ultrasonicated hydrogels

The following samples were prepared:

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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