User:Allison K. Alix/Notebook/CHEM-581/2013/03/29
 Experimental Chemistry
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
Objective-Centrifuge concentration gradient solutions to observe absorbance in each -Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH ProceduresPart 1: Preparing Concentration Gradients for UV-Vis Measurements 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. 2) Make the following stock solutions to compare the supernatant absorbances to:
3) Take UV-Vis spectra of stock solutions and supernatants Part 2: Observing pH changes 1) Separate crystal violet containing hydrogels into two epi-test tubes 2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other 3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom 4) Take UV-Vis of supernatants Part 3: Prepare hydrogels with indicators 1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye 2) Dissolve each in 1.5mL phosphate buffer. 3) Allow time for them to separate 4) Add Data
| |
