User:Allison K. Alix/Notebook/CHEM-581/2013/02/20

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Description)
(Procedures)
Line 32: Line 32:
Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 +
 +
*only done for 0.25g sample
Set 3:
Set 3:

Revision as of 10:11, 20 February 2013

Microgel Prep III Main project page
Previous entry      Next entry


Objective

  • Use Safflower Oil in place of Mineral oil
  • Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

Data

Notes


Personal tools