User:Allison K. Alix/Notebook/CHEM-581/2013/02/20

Objective

• Use Safflower Oil in place of Mineral oil

• Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

• only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

Data

Notes