User:Allison K. Alix/Notebook/CHEM-581/2013/02/20: Difference between revisions
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Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | ||
*done for all samples | |||
Set 2: | Set 2: | ||
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Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing | Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing | ||
*done for all samples | |||
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing | |||
==Data== | ==Data== | ||
Revision as of 08:52, 20 February 2013
 Microgel Prep III
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Objective
ProceduresMake the following samples: 1) 0.5g PVOH, 4 mL buffer, 50 mL oil 2) 0.25g PVOH, 3 mL buffer, 50 mL oil 3) 0.14g PVOH, 3mL buffer, 50 mL oil Set 1: Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Set 2: Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Set 3: Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing DataNotes | |
