User:Allison K. Alix/Notebook/CHEM-581/2013/02/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep III</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep III</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
* Use Safflower Oil | * Use Safflower Oil (higher freezing point than mineral oil) | ||
* Freeze using Liquid Nitrogen before placing in freezer | * Freeze using Liquid Nitrogen before placing in freezer | ||
== | ==Procedures== | ||
Make the following samples: | Make the following samples: | ||
| Line 25: | Line 25: | ||
3) 0.14g PVOH, 3mL buffer, 50 mL oil | 3) 0.14g PVOH, 3mL buffer, 50 mL oil | ||
Set 1: | |||
== | Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | ||
*done for all samples | |||
Set 2: | |||
Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | |||
*only done for 0.25g sample | |||
Set 3: | |||
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing | |||
*done for all samples | |||
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing | |||
==Observations== | |||
For Set 1, our purpose in using the petri dishes was to create a larger surface area for the hydrogels to form. After preparing our water/oil emulsion and placing in the freezer for ~10min, it was apparent that although there would be a greater separation between the gels that form, there would still be significant adhesion between them. Because of this, we decided to only to do one sample in the same way using mineral oil, just to see if there was any difference. For Set 3, we used safflower oil and flash froze our sample prior to placing in the freezer. This method was done on all three samples. | |||
Latest revision as of 22:28, 26 September 2017
 Microgel Prep III
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Objective
ProceduresMake the following samples: 1) 0.5g PVOH, 4 mL buffer, 50 mL oil 2) 0.25g PVOH, 3 mL buffer, 50 mL oil 3) 0.14g PVOH, 3mL buffer, 50 mL oil Set 1: Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Set 2: Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Set 3: Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing ObservationsFor Set 1, our purpose in using the petri dishes was to create a larger surface area for the hydrogels to form. After preparing our water/oil emulsion and placing in the freezer for ~10min, it was apparent that although there would be a greater separation between the gels that form, there would still be significant adhesion between them. Because of this, we decided to only to do one sample in the same way using mineral oil, just to see if there was any difference. For Set 3, we used safflower oil and flash froze our sample prior to placing in the freezer. This method was done on all three samples.
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