User:Allison K. Alix/Notebook/CHEM-581/2013/02/15

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Prep w/ new ratios/Crosslinking of Hydrogels</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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3) 0.14g PVOH, 3mL buffer, 50 mL oil
3) 0.14g PVOH, 3mL buffer, 50 mL oil
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==Data==
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In addition to this, we will cross link already formed hydrogels from [[User:Allison K. Alix/Notebook/CHEM-581/2013/02/01|02/01/2013]]
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* Add data and results here...
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==Procedures==
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1) Heat ~75mL gluteraldehyde fixant solution (contains 0.1M HCl and Na2SO4) to 60°C
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2) Place 5 hydrogels in solution with 1mL gluteraldehyde.
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3) Gradually take 1 hydrogel out every 15 minutes and store in water.
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4) Observe effects of crosslinkage
==Notes==
==Notes==
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This area is for any observations or conclusions that you would like to note.
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We have observed that our nanogels are sinking to the bottom of the beaker and clumping together during the freeze-thaw method. We are interested in changing a few variables. First, we will try flash freezing the solution before it is ready to be placed in the freezer for the first cycle using liquid nitrogen. This will freeze the gels in whatever position they may be in and will hopefully stop them from collecting at the bottom of the beaker. Second, we are interested in using an oil with a higher freezing point. The mineral oil we are using does not freeze very readily in the -20°C environment. Saffron oil will be our next choice.
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Course]]

Current revision

Microgel Prep w/ new ratios/Crosslinking of Hydrogels Main project page
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Objective

  • Vary the ratio of PVOH/Phosphate buffer to Mineral oil.

Description

We will look at the following solutions for preparing microgels:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

In addition to this, we will cross link already formed hydrogels from 02/01/2013

Procedures

1) Heat ~75mL gluteraldehyde fixant solution (contains 0.1M HCl and Na2SO4) to 60°C

2) Place 5 hydrogels in solution with 1mL gluteraldehyde.

3) Gradually take 1 hydrogel out every 15 minutes and store in water.

4) Observe effects of crosslinkage

Notes

We have observed that our nanogels are sinking to the bottom of the beaker and clumping together during the freeze-thaw method. We are interested in changing a few variables. First, we will try flash freezing the solution before it is ready to be placed in the freezer for the first cycle using liquid nitrogen. This will freeze the gels in whatever position they may be in and will hopefully stop them from collecting at the bottom of the beaker. Second, we are interested in using an oil with a higher freezing point. The mineral oil we are using does not freeze very readily in the -20°C environment. Saffron oil will be our next choice.



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