User:Allison K. Alix/Notebook/CHEM-581/2013/02/15

Objective

• Vary the ratio of PVOH/Phosphate buffer to Mineral oil.

Description

We will look at the following solutions for preparing microgels:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

In addition to this, we will cross link already formed hydrogels from 02/01/2013

Procedures

1) Heat ~75mL gluteraldehyde fixant solution (contains 0.1M HCl and Na2SO4) to 60°C

2) Place 5 hydrogels in solution with 1mL gluteraldehyde.

3) Gradually take 1 hydrogel out every 15 minutes and store in water.

4) Observe effects of crosslinkage

Notes

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