User:Allison K. Alix/Notebook/CHEM-581/2013/02/11

From OpenWetWare

Jump to: navigation, search
Preparing Microgels Part II Main project page
Previous entry      Next entry


Objective

  • Prepare smaller hydrogels using a similar procedure to the one used on 2/06/13

Description

The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale)

Procedures

1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution

2) Heat at 100°C for ~15 minutes

3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min

4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.

Observations

-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools