User:Allison K. Alix/Notebook/CHEM-581/2013/02/11: Difference between revisions
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The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale) | The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale) | ||
== | ==Procedures== | ||
1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution | |||
2) Heat at 100°C for ~15 minutes | |||
3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min | |||
4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles. | |||
==Notes== | ==Notes== |
Revision as of 07:11, 13 February 2013
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Objective
DescriptionThe nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale) Procedures1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution 2) Heat at 100°C for ~15 minutes 3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min 4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles. NotesThis area is for any observations or conclusions that you would like to note.
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