User:Allison K. Alix/Notebook/CHEM-581/2013/02/11

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Preparing Microgels Part II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.
4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.
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==Notes==
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==Observations==
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This area is for any observations or conclusions that you would like to note.
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-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels.
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Course]]

Current revision

Preparing Microgels Part II Main project page
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Objective

  • Prepare smaller hydrogels using a similar procedure to the one used on 2/06/13

Description

The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale)

Procedures

1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution

2) Heat at 100°C for ~15 minutes

3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min

4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.

Observations

-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels.



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