User:Allison K. Alix/Notebook/CHEM-581/2013/02/06 - Revision history

Allison K. Alix at 15:58, 6 February 2013

2013-02-06T15:58:14Z

←Older revision		Revision as of 15:58, 6 February 2013
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	{\|{{table}} width="800"		{\|{{table}} width="800"
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-	\|style="background-color: #EEE"\|[[Image:BDLlogo_notext_lr.png\|128px]]<span style="font-size:22px;"> ~~Experimental Chemistry~~</span>	+	\|style="background-color: #EEE"\|[[Image:BDLlogo_notext_lr.png\|128px]]<span style="font-size:22px;"> Microgel Preparation</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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2013-02-06T15:51:03Z

Calculations

←Older revision		Revision as of 15:51, 6 February 2013
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	[Base] = monosodium phosphate, 119.98 g/mol		[Base] = monosodium phosphate, 119.98 g/mol
		+
	[Acid] = disodium phosphate, 141.96 g/mol		[Acid] = disodium phosphate, 141.96 g/mol

2013-02-06T15:47:15Z

References

←Older revision		Revision as of 15:47, 6 February 2013
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-	Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.	+

	[[Category:Course]]		[[Category:Course]]

2013-02-06T15:46:18Z

Procedure

←Older revision		Revision as of 15:46, 6 February 2013
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	2) Dissolve 0.5 g PVOH (MW= 89,000-98,000 g/mol) in 3mL phosphate buffer. Heat @ 100°C for 15 minutes with stirring.		2) Dissolve 0.5 g PVOH (MW= 89,000-98,000 g/mol) in 3mL phosphate buffer. Heat @ 100°C for 15 minutes with stirring.
		+
		+	3) Add 2μL R6G in 2mL phosphate buffer to the PVOH/buffer mixture. Be sure to mix thoroughly.
		+
		+	4) Add 100mL Mineral Oil (CVS, 99.9%) to a homogenizer and subsequently add the PVOH mixture. Homogenize for ~5minutes to form a w/o emulsion
		+
		+	5) Pour final w/o emulsion into a beaker and place in a freezer at -20°C for 20 hours. Remove and allow thawing for 4 hours. Repeat this cycle for a total of three cycles.

	==Calculations==		==Calculations==

2013-02-06T14:39:52Z

Procedure

←Older revision		Revision as of 14:39, 6 February 2013
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	==Procedure==		==Procedure==
		+
		+	1) Prepare 20mM phosphate buffer (pH=7.4)
		+
		+	::Dissolve 1.256 g disodium phosphate and 1.337 g monosodium phosphate in ~900mL water. Add sodium hydroxide to obtain pH of 7.4 (~100mL)
		+
		+	2) Dissolve 0.5 g PVOH (MW= 89,000-98,000 g/mol) in 3mL phosphate buffer. Heat @ 100°C for 15 minutes with stirring.

	==Calculations==		==Calculations==

2013-02-06T14:36:32Z

Calculations

←Older revision		Revision as of 14:36, 6 February 2013
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	8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate		8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate
		+
		+	11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate

	==References==		==References==

2013-02-06T14:36:21Z

Procedure

←Older revision		Revision as of 14:36, 6 February 2013
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	==Procedure==		==Procedure==
-
-	~~'''Preparation of 20mM phosphate buffer, pH = 7.4'''~~
-
-	~~[Base] = monosodium phosphate, 119.98 g/mol~~
-	~~[Acid] = disodium phosphate, 141.96 g/mol~~
-
-	~~pH = pKa + log ([Base]/[Acid])~~
-
-	~~7.4 = 7.3 + log ([Base]/[Acid])~~
-
-	~~([Base]/[Acid]) = 1.2589~~
-
-	~~[Acid] + [Base] = 20mM~~
-
-	~~[Base] = 20mM - [Acid]~~
-
-	~~(20mM - [Acid])/[Acid] = 1.2589~~
-
-	~~[disodium phosphate] = 8.85mM~~
-
-	~~[monosodium phosphate] = 11.14mM~~
-
-	~~8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate~~
-
-	~~11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate~~

	==Calculations==		==Calculations==

2013-02-06T14:35:55Z

Calculations

←Older revision		Revision as of 14:35, 6 February 2013
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	==Calculations==		==Calculations==
-	* Add data and results here...	+
		+	'''Preparation of 20mM phosphate buffer, pH = 7.4'''
		+
		+	[Base] = monosodium phosphate, 119.98 g/mol
		+	[Acid] = disodium phosphate, 141.96 g/mol
		+
		+	pH = pKa + log ([Base]/[Acid])
		+
		+	7.4 = 7.3 + log ([Base]/[Acid])
		+
		+	([Base]/[Acid]) = 1.2589
		+
		+	[Acid] + [Base] = 20mM
		+
		+	[Base] = 20mM - [Acid]
		+
		+	(20mM - [Acid])/[Acid] = 1.2589
		+
		+	[disodium phosphate] = 8.85mM
		+
		+	[monosodium phosphate] = 11.14mM
		+
		+	8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate

	==References==		==References==

2013-02-06T14:35:29Z

Data

←Older revision		Revision as of 14:35, 6 February 2013
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	11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate		11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate

-	==~~Data~~==	+	==Calculations==
	* Add data and results here...		* Add data and results here...

2013-02-06T14:34:50Z

Procedure

←Older revision		Revision as of 14:34, 6 February 2013
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	[disodium phosphate] = 8.85mM		[disodium phosphate] = 8.85mM

-	[~~Base~~] = 11.14mM	+	[monosodium phosphate] = 11.14mM
		+
		+	8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate
		+
		+	11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate

	==Data==		==Data==