User:Allison K. Alix/Notebook/CHEM-581/2013/02/06

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Microgel Preparation</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Procedure==
==Procedure==
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1) Prepare 20mM phosphate buffer (pH=7.4)
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::Dissolve 1.256 g disodium phosphate and 1.337 g monosodium phosphate in ~900mL water. Add sodium hydroxide to obtain pH of 7.4 (~100mL)
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2) Dissolve 0.5 g PVOH (MW= 89,000-98,000 g/mol) in 3mL phosphate buffer. Heat @ 100°C for 15 minutes with stirring.
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3) Add 2μL R6G in 2mL phosphate buffer to the PVOH/buffer mixture. Be sure to mix thoroughly.
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4) Add 100mL Mineral Oil (CVS, 99.9%) to a homogenizer and subsequently add the PVOH mixture. Homogenize for ~5minutes to form a w/o emulsion
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5) Pour final w/o emulsion into a beaker and place in a freezer at -20°C for 20 hours. Remove and allow thawing for 4 hours. Repeat this cycle for a total of three cycles.
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==Calculations==
'''Preparation of 20mM phosphate buffer, pH = 7.4'''
'''Preparation of 20mM phosphate buffer, pH = 7.4'''
[Base] = monosodium phosphate, 119.98 g/mol
[Base] = monosodium phosphate, 119.98 g/mol
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[Acid] = disodium phosphate, 141.96 g/mol
[Acid] = disodium phosphate, 141.96 g/mol
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11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate
11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate
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==Data==
 
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* Add data and results here...
 
==References==
==References==
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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[[Category:Course]]
[[Category:Course]]

Current revision

Microgel Preparation Main project page
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Objective

-Begin PVA microsphere preparation

Procedure

1) Prepare 20mM phosphate buffer (pH=7.4)

Dissolve 1.256 g disodium phosphate and 1.337 g monosodium phosphate in ~900mL water. Add sodium hydroxide to obtain pH of 7.4 (~100mL)

2) Dissolve 0.5 g PVOH (MW= 89,000-98,000 g/mol) in 3mL phosphate buffer. Heat @ 100°C for 15 minutes with stirring.

3) Add 2μL R6G in 2mL phosphate buffer to the PVOH/buffer mixture. Be sure to mix thoroughly.

4) Add 100mL Mineral Oil (CVS, 99.9%) to a homogenizer and subsequently add the PVOH mixture. Homogenize for ~5minutes to form a w/o emulsion

5) Pour final w/o emulsion into a beaker and place in a freezer at -20°C for 20 hours. Remove and allow thawing for 4 hours. Repeat this cycle for a total of three cycles.

Calculations

Preparation of 20mM phosphate buffer, pH = 7.4

[Base] = monosodium phosphate, 119.98 g/mol

[Acid] = disodium phosphate, 141.96 g/mol

pH = pKa + log ([Base]/[Acid])

7.4 = 7.3 + log ([Base]/[Acid])

([Base]/[Acid]) = 1.2589

[Acid] + [Base] = 20mM

[Base] = 20mM - [Acid]

(20mM - [Acid])/[Acid] = 1.2589

[disodium phosphate] = 8.85mM

[monosodium phosphate] = 11.14mM

8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate

11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate

References

http://www.sciencedirect.com/science/article/pii/S0168365998000893



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