User:Allison K. Alix/Notebook/CHEM-581/2013/02/01
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< User:Allison K. Alix | Notebook | CHEM-581 | 2013 | 02(Difference between revisions)
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==Objective== | ==Objective== | ||
| - | Prepare hydrogels in smaller form | + | -Prepare hydrogels in smaller form |
| - | == | + | ==Procedure== |
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| + | 1) Prepare PVOH hydrogels using procedure from[[User:Allison_K._Alix/Notebook/CHEM-581/2013/01/30|1/30/2013]], with dye (1μL dye for every 1mL PVOH solution) | ||
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| + | 2) Distribute 300μL of each PVOH hydrogel solution into 1 well. | ||
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| + | 3) Freeze at 20°C for 24 hours. Thaw for 24 hours. Repeat this freeze thaw cycle for 3 total cycles | ||
==Data== | ==Data== | ||
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| - | + | [[Image:IMAG1368.jpg|center|400px]] | |
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[[Category:Course]] | [[Category:Course]] | ||
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Objective-Prepare hydrogels in smaller form Procedure1) Prepare PVOH hydrogels using procedure from1/30/2013, with dye (1μL dye for every 1mL PVOH solution) 2) Distribute 300μL of each PVOH hydrogel solution into 1 well. 3) Freeze at 20°C for 24 hours. Thaw for 24 hours. Repeat this freeze thaw cycle for 3 total cycles Data
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