User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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* To perform column chromatography on BPA solutions from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-581_Experimental_Chemistry_I/2014/10/22 Oct. 22] | * To perform column chromatography on BPA solutions from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-581_Experimental_Chemistry_I/2014/10/22 Oct. 22] | ||
===Preparation of packed | ===Preparation of packed column=== | ||
* Place glass wool on bottom of column | * Place glass wool on bottom of column | ||
* Cover with a layer of sand | * Cover with a layer of sand | ||
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* Allow to settle | * Allow to settle | ||
* Rinse with water to remove ethanol | * Rinse with water to remove ethanol | ||
===Concentration of 10.4 ppm BPA solution=== | |||
* Added 10 mL of BPA | |||
* Added 10 mL water | |||
* Added 30 mL of methanol | |||
*Collected eluate at 5 mL intervals | |||
* Spotted on TLC plate, and using UV light, checked for fluorescence. Water 2, Methanol 1 and methanol 2 culture tubes gloved faintly. | |||
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Latest revision as of 00:28, 27 September 2017
Project name | Main project page Previous entry Next entry |
Tasks for October 24
Preparation of packed column
Concentration of 10.4 ppm BPA solution
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