User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(fix raw html notebook nav)
 
(8 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 8: Line 8:
==Tasks for October 24==
==Tasks for October 24==
* To prepare packed columns
* To prepare packed columns
* To perform column chromatography on BPA solutions from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-581_Experimental_Chemistry_I/2014/10/22 Oct. 22]


===Preparation of packed chemicals===
===Preparation of packed column===
* Put glass wool on bottom
* Place glass wool on bottom of column
* Cover with a layer of sand
* Cover with a layer of sand
* Add silica packing material prepared by dissolved 10 g of silica in methanol
* Add silica packing material prepared by dissolved 10 g of silica in methanol
* Allow to settle
* Allow to settle
* Add a layer of sand at the top to avoid disturbance of packing column
* Rinse with water to remove ethanol


===Concentration of 10.4 ppm BPA solution===
* Added 10 mL of BPA
* Added 10 mL water
* Added 30 mL of methanol
*Collected eluate at 5 mL intervals


* Spotted on TLC plate, and using UV light, checked for fluorescence. Water 2, Methanol 1 and methanol 2 culture tubes gloved faintly.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 00:28, 27 September 2017

Project name Main project page
Previous entry      Next entry

Tasks for October 24

  • To prepare packed columns
  • To perform column chromatography on BPA solutions from Oct. 22

Preparation of packed column

  • Place glass wool on bottom of column
  • Cover with a layer of sand
  • Add silica packing material prepared by dissolved 10 g of silica in methanol
  • Allow to settle
  • Rinse with water to remove ethanol

Concentration of 10.4 ppm BPA solution

  • Added 10 mL of BPA
  • Added 10 mL water
  • Added 30 mL of methanol
  • Collected eluate at 5 mL intervals
  • Spotted on TLC plate, and using UV light, checked for fluorescence. Water 2, Methanol 1 and methanol 2 culture tubes gloved faintly.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Alicia_Rasines_Mazo/Notebook/CHEM-581_Experimental_Chemistry_I/2014/10/24&oldid=1015705"