User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/01: Difference between revisions

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#**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out)
#**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out)
#*** 2μM stock concentration: (2500μM)v<sub>1</sub>=(2.0μM)(5mL);  '''v<sub>1</sub>'''=0.004mL
#*** 2μM stock concentration: (2500μM)v<sub>1</sub>=(2.0μM)(5mL);  '''v<sub>1</sub>'''=0.004mL
#** 500μM dilution: (2500μM)v<sub>1</sub>=(500μM)(5mL);  '''v<sub>1</sub>'''=1mL of 2500μM R6G stock solution
# Take UV-Vis and Fluorescence spectra of these samples.
# Take UV-Vis and Fluorescence spectra of these samples.
# Make a calibration curve based on UV-Vis.
# Make a calibration curve based on UV-Vis.

Revision as of 05:50, 1 October 2014

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Task for October 1

  • To determine fluorophore concentration

Fluorescence

Procedure followed as directed by Dr. Hartings on Oct. 1<br.>

  1. Made stock concentrations of Rhodamine 6G (both groups can use the same solutions)
    1. 0.10μM
    2. 0.50μM
    3. 1.0μM
    4. 1.5μM
    5. 2.0μM
    • Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared, this solution was further diluted to 500μM to make rest of solutions, except for 2.0 μM, which was prepared using 2500μM stock solution
      • (2500×10-6mol/L)*(0.010L)*(474.02g/mol)=0.01185 g (0.0120 g of R6G were measured out)
        • 2μM stock concentration: (2500μM)v1=(2.0μM)(5mL); v1=0.004mL
      • 500μM dilution: (2500μM)v1=(500μM)(5mL); v1=1mL of 2500μM R6G stock solution
  2. Take UV-Vis and Fluorescence spectra of these samples.
  3. Make a calibration curve based on UV-Vis.
    1. Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    1. In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.


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