User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/01: Difference between revisions

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* To determine fluorophore concentration
* To determine fluorophore concentration
===Fluorescence===
===Fluorescence===
Procedure followed as directed by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/10/01Dr. Hartings on Oct. 1]<br.>
Procedure followed as directed by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/10/01 Dr. Hartings on Oct. 1]<br.>
 
<u>Fluorescence</u>
# Make stock concentrations (both groups can use the same solutions)
## 0.10uM
## 0.50uM
## 1.0uM
## 1.5uM
## 2.0uM
# Take UV-Vis and Fluorescence spectra of these samples.
# Make a calibration curve based on UV-Vis.
## Compare your data to some published values
# Make a calibration curve based on the fluorescence.
## In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.
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Revision as of 05:13, 1 October 2014

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Task for October 1

  • To determine fluorophore concentration

Fluorescence

Procedure followed as directed by Dr. Hartings on Oct. 1<br.> Fluorescence

  1. Make stock concentrations (both groups can use the same solutions)
    1. 0.10uM
    2. 0.50uM
    3. 1.0uM
    4. 1.5uM
    5. 2.0uM
  2. Take UV-Vis and Fluorescence spectra of these samples.
  3. Make a calibration curve based on UV-Vis.
    1. Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    1. In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.


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