User:Alicia Rasines Mazo/Notebook/CHEM-572 Experimental Biological Chemistry II/2015/01/27: Difference between revisions
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* Formation of 30:1 Gold-Lysozyme nanoparticle colloid | * Formation of 30:1 Gold-Lysozyme nanoparticle colloid | ||
* Formation of 45:1 Gold-Lysozyme fibers | * Formation of 45:1 Gold-Lysozyme fibers | ||
===Preparation of stock solutions=== | |||
*50 mL of 2.512mM HAuCl4 stock solution was prepared by adding 0.0427 g of HAuCl4 in distilled water. | |||
**(0.05L)(2.512×10<sup>-3</sup> mol/L)(340 g/mol)=0.0427 g | |||
*50 mL of 20 μM lysozyme stock solution was prepared by adding 0.014307 g of Lysozyme in distilled water | |||
**((0.05L)(20×10<sup>-6</sup> mol/L)(14307 g/mol)= 0.014307 g | |||
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs. | |||
*Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 250 μM) | |||
* Also add 2.075 mL of 20μM of lysozyme stock | |||
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
**8.3×10<sup>-6</sup>(5mL)=20×10<sup>-6</sup>v<sub>2</sub> | |||
**v<sub>2</sub>=2.075 mL | |||
*Make up to 5 mL with distilled water | |||
Revision as of 11:23, 27 January 2015
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Tasks for January 27
Preparation of stock solutions
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs.
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