User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/28

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Tasks for October 28

  • To continue analysis of dialysed 0.6 g/ L Lysozyme vs KI using 3500 MWCO
  • To check I- ISE values obtained on OCt. 21 and measure KI content of dialysed solutions
  • Set up dialysis of 0.6 g/L lysozyme vs CaCl2 using 3500 MWCO

I- ISE

KI concentration mV measurement (mV) (21 Oct.) mV measurement (mV) (28 Oct.)
2mM -198.3 -192.8
5 mM -217.3 -206.8
10 mM -237.0 -224.2
25 mM -247.4 -253.0
50mM -278.0 -276.2

0.6 g/L lysozyme vs CaCl2 using 3500 MWCO dialysis data

Ca2+ ISE

CaCl2 concentration mV measurement (mV)
5μM (1) 11.8
Lys (1) 4.6
50μM (2) 4.2
Lys (2) 0.1
500μM (3) 27.4
Lys (3) 24.4
5mM (4) 52.1
Lys (4) 51.3
50mM (5) 77.3
Lys (5) 77.0
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