User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/28

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Tasks for October 28

  • To continue analysis of dialysed 0.6 g/ L Lysozyme vs KI using 3500 MWCO
  • To check I- ISE values obtained on OCt. 21 and measure KI content of dialysed solutions
  • Set up dialysis of 0.6 g/L lysozyme vs CaCl2 using 3500 MWCO

I- ISE

KI concentration mV measurement (mV) (21 Oct.) mV measurement (mV) (28 Oct.)
2mM -198.3 -192.8
5 mM -217.3 -206.8
10 mM -237.0 -224.2
25 mM -247.4 -253.0
50mM -278.0 -276.2

0.6 g/L lysozyme vs CaCl2 using 3500 MWCO dialysis data

Ca2+ ISE

CaCl2 concentration mV measurement (mV)
5μM (1) 12.9
Lys (1)
50μM (2)
Lys (2)
500μM (3)
Lys (3)
5mM (4)
Lys (4)
50mM (5)
Lys (5)
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