User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/28: Difference between revisions

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*To continue analysis of dialysed 0.6 g/ L Lysozyme vs KI using 3500 MWCO
*To continue analysis of dialysed 0.6 g/ L Lysozyme vs KI using 3500 MWCO
* To check I<sup>-</sup> ISE values obtained on [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/21 OCt. 21] and measure KI content of dialysed solutions
* To check I<sup>-</sup> ISE values obtained on [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/21 OCt. 21] and measure KI content of dialysed solutions
* Set up dialysis of 0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO


<u>I- ISE</u>
<u>I- ISE</u>
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|-
|-
| 50mM || -278.0 || -276.2
| 50mM || -278.0 || -276.2
|-
|}
===0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO dialysis data===
<u>Ca2+ ISE</u>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> concentration'''
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)'''
|-
| 5μM (1) ||
|-
| Lys (1) ||
|-
| 50μM (2) ||
|-
| Lys (2) ||
|-
| 500μM (3) ||
|-
| Lys (3) ||
|-
| 5mM (4) ||
|-
| Lys (4) ||
|-
| 50mM (5)||
|-
| Lys (5) ||
|-
|-
|}
|}
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Revision as of 09:36, 29 October 2014

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Tasks for October 28

  • To continue analysis of dialysed 0.6 g/ L Lysozyme vs KI using 3500 MWCO
  • To check I- ISE values obtained on OCt. 21 and measure KI content of dialysed solutions
  • Set up dialysis of 0.6 g/L lysozyme vs CaCl2 using 3500 MWCO

I- ISE

KI concentration mV measurement (mV) (21 Oct.) mV measurement (mV) (28 Oct.)
2mM -198.3 -192.8
5 mM -217.3 -206.8
10 mM -237.0 -224.2
25 mM -247.4 -253.0
50mM -278.0 -276.2

0.6 g/L lysozyme vs CaCl2 using 3500 MWCO dialysis data

Ca2+ ISE

CaCl2 concentration mV measurement (mV)
5μM (1)
Lys (1)
50μM (2)
Lys (2)
500μM (3)
Lys (3)
5mM (4)
Lys (4)
50mM (5)
Lys (5)
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