User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/28: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(fix raw html notebook nav) |
||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 27: | Line 27: | ||
|- | |- | ||
|} | |} | ||
[[Image:I calibration curve 281014.png]] <br.> | |||
Calibration curve for iodine obtained on Oct. 28. | |||
===0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO dialysis data=== | ===0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO dialysis data=== | ||
<u>Bradford Analysis</u> | <u>Bradford Analysis</u> | ||
Line 62: | Line 63: | ||
|} | |} | ||
<u>UV-Vis Absorption</u> | <u>UV-Vis Absorption</u> | ||
<u>UV-Vis Absorption</u> <br.> | |||
Transfer 100 μL of 100:1 diluted dialysed lysozume to a small volume UV cuvette & measure UV absorption | |||
**Be sure your cuvette is clean before hand. Use SDS, HCl, HPLC, & methanol washes | |||
**Measured entire 200 - 800 nm range. (Care about peak at 280 nm. Need to compare to fluorescence data to see how the binding is affected) | |||
<u>Fluorescence</u> | <u>Fluorescence</u> | ||
<u>Fluorescence</u> | <u>Fluorescence</u> |
Latest revision as of 00:28, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||
Tasks for October 28
I- ISE
<br.> Calibration curve for iodine obtained on Oct. 28. 0.6 g/L lysozyme vs CaCl2 using 3500 MWCO dialysis dataBradford Analysis
Ca2+ ISE
UV-Vis Absorption UV-Vis Absorption <br.> Transfer 100 μL of 100:1 diluted dialysed lysozume to a small volume UV cuvette & measure UV absorption
Fluorescence Fluorescence
|