User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(fix raw html notebook nav) |
||
(6 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
==Tasks for October 22== | ==Tasks for October 22== | ||
* Data analysis for 30:1 colloid solution vs CaCl2 using MWCO 3500 | * Data analysis for 30:1 colloid solution vs CaCl2 using MWCO 3500 | ||
* New dialysis of 0.6 g/ L Lysozyme vs KI using 3500 MWCO since dialysis from [http://openwetware.org/wiki/User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/08 Oct. 8] did not work due to incorrect dilutions | |||
===0.6g/L solution vs KI (MWCO 3500) Dialysis Data=== | |||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | |||
<u>Bradford Analysis</u> | |||
*Only running bradford analysis of protein-containing solutions, that is Lys 1, 2, 3, 4 and 5, since the protein cannot diffuse through 3500 MWCO. | |||
* Remove 50 μL of solution from each chamber (7 in all) and run Bradford analysis | |||
**Bradford reagent should be diluted 1:3 with 50mM Tris/50mM NaCl | |||
**Recall, 50 μL solution + 200 μL diluted Bradford + 750 μL Tris/NaCl buffer | |||
**PS cuvettes, measuring 400 - 800 nm | |||
[[Image:Bradford assay 281014.png]] | |||
<u>I- ISE</u> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''I<sup>-</sup> concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| 2mM (1) || -175.3 | |||
|- | |||
| Lys (1) || -174.0 | |||
|- | |||
| 5 mM || -196.4 | |||
|- | |||
| Lys (2) || -188.5 | |||
|- | |||
| 10 mM || -208.9 | |||
|- | |||
| Lys (3) || -210.5 | |||
|- | |||
| 25 mM || -231.5 | |||
|- | |||
| Lys (4) || -229.1 | |||
|- | |||
| 50 mM || -250.2 | |||
|- | |||
| Lys (5) || -244.3 | |||
|- | |||
|} | |||
<u>UV-Vis measurements</u> | |||
* It was found that KI interferes with the Bradford reagent, so that no Bradford can bind to protein. No peak was seen using UV-Vis | |||
<u>Fluorescence measurements</u> | |||
* No fluorescence measurements were taken on the samples, since nothing was detected using UV-Vis absorption. | |||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
Latest revision as of 00:28, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||
Tasks for October 22
0.6g/L solution vs KI (MWCO 3500) Dialysis DataBradford Analysis
I- ISE
UV-Vis measurements
Fluorescence measurements
|