User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/21: Difference between revisions
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===30:1 colloid solution vs CaCl2 (MWCO 3500) Dialysis Data=== | ===30:1 colloid solution vs CaCl2 (MWCO 3500) Dialysis Data=== | ||
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<u>Bradford Analysis</u> | |||
*Only running bradford analysis of protein-containing solutions, that is Colloid 1, 2, 3, 4 and 5, since the protein cannot diffuse through 3500 MWCO. | |||
* Remove 100 μL of solution from each chamber (7 in all) and run Bradford analysis | |||
**Bradford reagent should be diluted 1:3 with 50mM Tris/50mM NaCl | |||
**Recall, 100 μL solution + 200 μL diluted Bradford + 700 μL Tris/NaCl buffer | |||
**PS cuvettes, measuring 400 - 800 nm | |||
<u>Ca2+ ISE</u> | <u>Ca2+ ISE</u> | ||
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Revision as of 11:47, 22 October 2014
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Tasks for Oct. 21
I- ISE preparationProdecure as describer by Dr. Fox on Oct. 21
I- ISE
30:1 colloid solution vs CaCl2 (MWCO 3500) Dialysis DataBradford Analysis
Ca2+ ISE
UV-Vis measurements
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