User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/21: Difference between revisions

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***The Ag/AgI wire can be connected directly to a multimeter using alligator clips
***The Ag/AgI wire can be connected directly to a multimeter using alligator clips
***You will continue to connect your Ag wire to the red lead on the multimeter
***You will continue to connect your Ag wire to the red lead on the multimeter
===30:1 colloid solution vs CaCl2 (MWCO 3500) Dialysis Data===
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Revision as of 09:29, 22 October 2014

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Tasks for Oct. 21

  • To prepare new dialysis of 30:1 colloid solution vs CaCl2 using MWCO 3500
  • To prepare iodide ion selective electrodes
  • To determine the molecular weight of the gel from electrophoresis started on Oct. 15

I- ISE preparation

Prodecure as describer by Dr. Fox on Oct. 21

    • Prepare 10 mL 3 M NaCl
      • Carefully remove yellow plastic covering on Ag/AgCl electrode (as instructed)
      • Place electrode in 15 mL falcon tube with enough NaCl solution to match volume in electrode
      • cover and store upright until needed
    • Prepare 50 mL 4 M KNO3
      • Add KNO3 to outer double junction cell, filling narrow tube + ~ 0.5 inches further
      • Place electrode in small vial with enough KNO3 solution to cover the vycor tip
    • Prepare 50 mL 0.01 M KI + 0.1 M KNO3
      • Cut a short piece (~ 3 in) of silver wire
      • Place in KI/KNO3 solution
      • Electrolyze the solution using the power supply
      • The standard reduction potential for Ag/AgI is -0.15 V
      • Oxidation occurs at the anode, which is connected to the red lead
      • Electrolyze at low power (low current) until the wire appears coated
    • Test your electrodes with a range of KI solutions
      • The Ag/AgCl electrode slides inside of the KNO3 bridge solution (this is a double junction electrode)
      • The Ag/AgI wire can be connected directly to a multimeter using alligator clips
      • You will continue to connect your Ag wire to the red lead on the multimeter

30:1 colloid solution vs CaCl2 (MWCO 3500) Dialysis Data
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