User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/15: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(fix raw html notebook nav) |
||
(4 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 18: | Line 18: | ||
*** Well 6: 0.6 g/L Lysozyme | *** Well 6: 0.6 g/L Lysozyme | ||
** Run as previously described. Refer to [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Harting's protocol]. | ** Run as previously described. Refer to [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Harting's protocol]. | ||
===Determination of molecular weight=== | |||
*By comparison with Precision Plus Protein Standards, the molecular weight of all samples was close to 15 KD, slightly lower, as expected for lysozyme compounds with MW=14,300 KD. | |||
* There was a very small quantity of unknown A and colloid in the gel. The very faint mark observed in well 3 could even be considered to be bleed-over from the 30:1 colloid in well 4. Therefore, electrophoresis was inclonclusive with respect to the molecular weight of unknown A. | |||
[[Image:Electrophoresis gel 151014.jpg]] | |||
[[Image:Precision Plus Protein.jpeg|400x300px|]] | |||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
Latest revision as of 00:26, 27 September 2017
Project name | Main project page Previous entry Next entry |
Tasks for October 15
SDS-PAGE
Determination of molecular weight
|