User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/08: Difference between revisions
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**Don't forget to run a blank with just Bradford & buffer | **Don't forget to run a blank with just Bradford & buffer | ||
**Don't forget to run your undialyzed 0.6 g/L Lysozyme stock | **Don't forget to run your undialyzed 0.6 g/L Lysozyme stock | ||
[[Image:Bradford analysis 081014.png]] | |||
<u>Bradford Calibration curve</u> | <u>Bradford Calibration curve</u> |
Revision as of 18:40, 15 October 2014
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Tasks for October 8
[I-] by Titration PrecipitationProcedure followed as detailed by Dr. Fox
Fluorescence of protein solutions
Preparation of new Dialysis with 0.6 g/ L Lysozyme vs KI using 3500 MWCO
Dialysis DataBradford Analysis
Bradford Calibration curve
<br.> UV-Vis Absorption <br.> Transfer 100 μL to a small volume UV cuvette & measure UV absorption
Fluorescence
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