User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/08: Difference between revisions
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*Did Bradford analysis for undialysed lysozyme solutions with concentrations 0.12, 0.3, 0.5, 0.6 and 1 g/L. | *Did Bradford analysis for undialysed lysozyme solutions with concentrations 0.12, 0.3, 0.5, 0.6 and 1 g/L. | ||
*The calibration curve below: | *The calibration curve below: | ||
[[Image:Lysozyme-Bradford CV.png]] | |||
<u>UV-Vis Absorption</u> <br.> | <u>UV-Vis Absorption</u> <br.> | ||
Transfer 100 μL to a small volume UV cuvette & measure UV absorption | Transfer 100 μL to a small volume UV cuvette & measure UV absorption |
Revision as of 09:29, 15 October 2014
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Tasks for October 8
[I-] by Titration PrecipitationProcedure followed as detailed by Dr. Fox
Fluorescence of protein solutions
Preparation of new Dialysis with 0.6 g/ L Lysozyme vs KI using 3500 MWCO
Dialysis DataBradford Analysis
Bradford Calibration curve
UV-Vis Absorption <br.> Transfer 100 μL to a small volume UV cuvette & measure UV absorption
Fluorescence
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