User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/08: Difference between revisions
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<u>Bradford Analysis</u> | <u>Bradford Analysis</u> | ||
*Only running bradford analysis of protein-containing solutions, that is Lys 1, 2, 3, 4 and 5, since the protein cannot diffuse through 3500 MWCO. | *Only running bradford analysis of protein-containing solutions, that is Lys 1, 2, 3, 4 and 5, since the protein cannot diffuse through 3500 MWCO. | ||
* Remove 20 μL of solution from each chamber (10 in all) and run Bradford analysis | |||
**Bradford reagent should be diluted 1:3 with 50mM Tris/50mM NaCl | |||
**Recall, 20 μL solution + 200 μL diluted Bradford + 780 μL Tris/NaCl buffer | |||
**PS cuvettes, measuring 400 - 800 nm | |||
**Don't forget to run a blank with just Bradford & buffer | |||
**Don't forget to run your undialyzed Lysozyme stock | |||
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Revision as of 09:58, 14 October 2014
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Tasks for October 8
[I-] by Titration PrecipitationProcedure followed as detailed by Dr. Fox
Fluorescence of protein solutions
Preparation of new Dialysis with 0.6 g/ L Lysozyme vs KI using 3500 MWCO
Dialysis DataBradford Analysis
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