User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*** [KI]= 0.0000493 mol KI / 0.001 L KI = '''49.3 mM KI''' | *** [KI]= 0.0000493 mol KI / 0.001 L KI = '''49.3 mM KI''' | ||
Titrations were performed as follows: | |||
* 9.68 mM NH<sub>4</sub>SCN was titrated against 3 mL 1M HNO<sub>3</sub>, 10 mL deionized H<sub>2</sub>O , 200 μLFerric alum indicator, and 0.5 mL of dialyzed sample | * 9.68 mM NH<sub>4</sub>SCN was titrated against 3 mL 1M HNO<sub>3</sub>, 10 mL deionized H<sub>2</sub>O , 200 μLFerric alum indicator, and 0.5 mL of dialyzed sample | ||
** Lysozyme against 2 mM KI | ** Lysozyme against 2 mM KI |
Latest revision as of 00:23, 27 September 2017
Project name | Main project page Previous entry Next entry |
Tasks for October 8
[I-] by Titration PrecipitationProcedure followed as detailed by Dr. Fox
Fluorescence of protein solutions
Preparation of new Dialysis with 0.6 g/ L Lysozyme vs KI using 3500 MWCO
Dialysis DataBradford Analysis
Bradford Calibration curve
<br.> UV-Vis Absorption <br.> Transfer 100 μL to a small volume UV cuvette & measure UV absorption
Fluorescence
[I-] titration
Titrations were performed as follows:
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