User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/30: Difference between revisions
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[[Image:CalciumCC.png]]<br.> | [[Image:CalciumCC.png]]<br.> | ||
**Try measuring 50 μM and 50 mM of other salts to check for interferences (Mg<sup>2+</sup> should, Na<sup>+</sup> should not) | **Try measuring 50 μM and 50 mM of other salts to check for interferences (Mg<sup>2+</sup> should, Na<sup>+</sup> should not) | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''Cu(II)Cl<sub>2</sub> concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''KCl concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | |||
|- | |||
| 50mM || 0||50mM||86.4 | |||
|- | |||
| 50mM || 50mM||0||80.5 | |||
|} | |||
'''Note''': There may be minimal interference by Cl<sup>-</sup> | |||
**Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | **Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | ||
{| {{table}} | {| {{table}} | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | | align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''NaSO<sub>4</sub>''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>HPO<sub>4</sub><sup>2-</sup>''' | ||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | | align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | ||
|- | |- | ||
| 50mM || 50 mM||0||- | | 50mM || 50 mM||0||-27.0 | ||
|- | |- | ||
| 50mM || 0||50mM||- | | 50mM || 0||50mM||-30.8 | ||
|- | |- | ||
|} | |} | ||
Revision as of 12:28, 1 October 2014
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Tasks for September 30
Making up calibration curves for Ca2+ and Cl-Procedure followed as described by Dr. Fox
Note: There may be minimal interference by Cl-
Dyalisis
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