User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/30: Difference between revisions
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[[Image:CalciumCC.png]]<br.> | |||
**Try measuring 50 μM and 50 mM of other salts to check for interferences (Mg<sup>2+</sup> should, Na<sup>+</sup> should not) | **Try measuring 50 μM and 50 mM of other salts to check for interferences (Mg<sup>2+</sup> should, Na<sup>+</sup> should not) | ||
**Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | **Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | ||
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[[Image:ClCC.png]] | |||
**Try measuring 50 μM and 50 mM of other salts to check for interferences & ion-pairing | **Try measuring 50 μM and 50 mM of other salts to check for interferences & ion-pairing | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | | align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | ||
| align="center" style="background:#f0f0f0;"|'''Cu(II)Cl<sub>2</sub> concentration''' | | align="center" style="background:#f0f0f0;"|'''Cu(II)Cl<sub>2</sub> concentration''' | ||
| align="center" style="background:#f0f0f0;"|'''KCl concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | | align="center" style="background:#f0f0f0;"|'''mV measurement (mV)''' | ||
|- | |- | ||
| | | 50mM || 50 mM||0||-7.7 | ||
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| 50μM || 50 μM||0||111.1 | |||
|- | |||
| 50mM || 0||50mM||-43.3 | |||
|- | |||
| 50μM || 0||50μM||115.6 | |||
|} | |} | ||
**Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | **Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution. | ||
{| {{table}} | {| {{table}} |
Revision as of 10:45, 1 October 2014
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Tasks for September 30
Making up calibration curves for Ca2+ and Cl-Procedure followed as described by Dr. Fox
Dyalisis
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