User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/23: Difference between revisions
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#* Repeat this step with fresh destain solution 2 more times | #* Repeat this step with fresh destain solution 2 more times | ||
===Bradford analysis for protein content=== | ===Bradford analysis for protein content=== | ||
*Both for dialyzed and non-dyalized BSA colloid solutions |
Revision as of 11:23, 23 September 2014
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Task for September 2014
Procedure followed as described by Dr. Hartings on Sept. 25 2013 ElectrophoresisPrepare the Gel and Assemble the Electrophoresis Cell
Note: 200mL of running buffer (10x SDS-PAGE running buffer 30.3 g Tris, 144.1g Clycine, 10g SDS, water to 1L) were diluted to 2 L. This amount was necessary in order to fill up the inner and outer buffer chambers Prepare and Load Samples
Perform Electrophoresis
Develop/Stain your gel
Bradford analysis for protein content
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