User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/23

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MATA2 Amplification on Chelex Extraction

EX Takara RXN Mixture conc 1X 3
Milli Q water 6.15 18.45
Takara EX Buffer 10X 1 3
dNTP 2.5uM 0.8 2.4
Primer Mix 5uM 1 3
EX Taq 5U 0.05 0.15
Template Chelex Extraction 1
27
9.0ul / RXN and 1.0ul Chelex Extraction
Genus species NCYC # sample
Schizosaccharomyces pombe 2722 1
Saccharomyces cerevisiae 1022 2
PCR cycle
98C 10 sec
60C 1 min

Load 2.0ul


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