Alien Genes New Primer Screen
Screened on CCA3
Reaction mixture
|
conc.
|
1x
|
16
|
Template |
1ng/ul |
1 |
16
|
TopTaq buffer |
10x |
1 |
16
|
dNTP mix |
10 mM each |
0.2 |
3.2
|
Primer mix |
5 uM each |
0.5 |
|
TopTaq (QIAGEN) |
5U/ul |
0.05 |
0.8
|
Milli Q |
|
7.25 |
116
|
|
|
10 ul |
|
PCR cycle |
|
|
|
94C |
3 min |
|
|
|
|
|
|
98C |
10 sec |
|
|
60C |
3 min |
|
|
x35 |
|
|
|
|
|
|
|
72C |
5 min |
|
|
|
Loaded 1.0ul of sample
AMP Lane
|
Primer
|
1 |
AV10004
|
2 |
AV10008
|
3 |
AV10010
|
4 |
AV10020
|
5 |
AV10022
|
6 |
AV10029
|
7 |
AV10032
|
8 |
AV10040.1
|
9 |
AV10040.2
|
10 |
AV10047
|
11 |
AV10049
|
12 |
AV10053
|
13 |
AV10087
|
14 |
AV10095
|
15 |
AV10114
|
|
Alien Genes New Primer Screen
Lane
|
Gene
|
Lane
|
Gene
|
1 |
AV10158 |
17 |
AV10084
|
2 |
AV10153 |
18 |
AV10082
|
3 |
AV10151 |
19 |
AV10075
|
4 |
AV10150 |
20 |
AV10071
|
5 |
AV10140 |
21 |
AV10045
|
6 |
AV10134 |
22 |
AV10043
|
7 |
AV10130 |
23 |
AV10042
|
8 |
AV10123 |
24 |
AV10041
|
9 |
AV10121 |
25 |
AV10034
|
10 |
AV10104 |
26 |
AV10028
|
11 |
AV10109 |
27 |
AV10027
|
12 |
AV10099 |
28 |
AV10025
|
13 |
AV10098 |
29 |
AV10016
|
14 |
AV10094 |
30 |
AV10011
|
15 |
AV10092 |
31 |
AV10007
|
16 |
AV10089 |
32 |
AV10002
|
|
Reaction mixture
|
conc.
|
1x
|
x33
|
Temp (Ithaca1 B5,C1,D1) |
?? |
2 |
66
|
TopTaq buffer |
10x |
1 |
33
|
dNTP mix |
10 mM each |
0.2 |
6.6
|
Primer mix |
5 uM each |
0.5 |
|
TopTaq (QIAGEN) |
5U/ul |
0.05 |
1.65
|
Milli Q |
|
6.25 |
206.25
|
|
|
10 ul |
|
PCR cycle |
|
|
|
94C |
3 min |
|
|
|
|
|
|
98C |
10 sec |
|
|
60C |
3 min |
|
|
x35 |
|
|
|
|
|
|
|
72C |
5 min |
|
|
|
Loaded 2.0ul of sample
Reaction mixture
|
conc.
|
1x
|
x33
|
Temp (Ithaca1 D3,D4,D6) |
?? |
2 |
66
|
TopTaq buffer |
10x |
1 |
33
|
dNTP mix |
10 mM each |
0.2 |
6.6
|
Primer mix |
5 uM each |
0.5 |
|
TopTaq (QIAGEN) |
5U/ul |
0.05 |
1.65
|
Milli Q |
|
6.25 |
206.25
|
|
|
10 ul |
|
PCR cycle |
|
|
|
94C |
3 min |
|
|
|
|
|
|
98C |
10 sec |
|
|
60C |
3 min |
|
|
x35 |
|
|
|
|
|
|
|
72C |
5 min |
|
|
|
Loaded 2.0ul of sample
Reaction mixture
|
conc.
|
1x
|
x33
|
Temp (Ithaca2 A1,A2,A3) |
?? |
2 |
66
|
TopTaq buffer |
10x |
1 |
33
|
dNTP mix |
10 mM each |
0.2 |
6.6
|
Primer mix |
5 uM each |
0.5 |
|
TopTaq (QIAGEN) |
5U/ul |
0.05 |
1.65
|
Milli Q |
|
6.25 |
206.25
|
|
|
10 ul |
|
PCR cycle |
|
|
|
94C |
3 min |
|
|
|
|
|
|
98C |
10 sec |
|
|
60C |
3 min |
|
|
x35 |
|
|
|
|
|
|
|
72C |
5 min |
|
|
|
Loaded 2.0ul of sample
|