User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/24

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To do

  • Extractions of A. vaga
  • test extraction volumes

Extraction

  • 10, 20, 40 A. vaga in 200 ul GeneMatrix
  • standard heating scheme, with periodic agitation during extraction

PCR

Reagents 1x conc. 4
h2o 5.75 - 23
10x 1 10x 4
dye 1 10x 4
dNTP 0.2 10 mM 0.8
Primers 0 5 uM 4
Taq 0.05 5 U/um 0.2
DNA 2 5 ng/ul
TOTAL 10 Cocktail per vial 9
  • 3m 95C (10s 95C, 1m 60C)x35
+ (40 ng) 10 20 40
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