User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/09: Difference between revisions
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===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C=== | ===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C=== | ||
*Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated. | |||
*Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects. | |||
[[Image:4.9.conductivity.4.png|800px|]] | [[Image:4.9.conductivity.4.png|800px|]] | ||
===Atomic Absorption Preparation=== | ===Atomic Absorption Preparation=== | ||
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==Figures== | ==Figures== | ||
===Atomic Absorption=== | ===Atomic Absorption=== | ||
*In comparison to yesterday this AA data has a more recognizable trend. Overall it appears that the amount of gold in solution decreases as the concentration of variable increases. | |||
[[Image:4.9.aa.hot.png|700px|]] | [[Image:4.9.aa.hot.png|700px|]] | ||
* 2,2 bipyridine data was just as wonky as yesterday by having an increase in gold followed by a prompt and steep decrease. | |||
[[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | [[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | ||
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[[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | [[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | ||
[[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | [[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | ||
*Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 15:23, 17 April 2014
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Objective
ProcedureConductivity Measurements of 30:1 Lysozyme-AuNP at 4°C
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:
FiguresAtomic Absorption
UV-Vis Images
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