User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/09: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | ==Objective== | ||
* | * Take conductivity measurements on 4°C solutions from 3/26/14. | ||
* Run UV-Vis and AA on solutions at 36°C from 3/26/14. | |||
==Procedure== | |||
===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C=== | |||
*Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated. | |||
*Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects. | |||
[[Image:4.9.conductivity.4.png|800px|]] | |||
===Atomic Absorption Preparation=== | |||
'''Creating the Gold Stock Solutions''' | |||
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio at 36°C were run on the AA: | |||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | |||
==Figures== | |||
===Atomic Absorption=== | |||
*In comparison to yesterday this AA data has a more recognizable trend. Overall it appears that the amount of gold in solution decreases as the concentration of variable increases. | |||
[[Image:4.9.aa.hot.png|700px|]] | |||
* 2,2 bipyridine data was just as wonky as yesterday by having an increase in gold followed by a prompt and steep decrease. | |||
[[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | |||
==UV-Vis Images== | ==UV-Vis Images== | ||
*Please note that very few samples actually had suspended nanoparticles in solution. | |||
[[Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png]] | [[Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png]] | ||
[[Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png]] | [[Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png]] | ||
[[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | [[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | ||
[[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | [[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | ||
*Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 15:23, 17 April 2014
Biomaterials Design Lab: Spring 2014 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
ProcedureConductivity Measurements of 30:1 Lysozyme-AuNP at 4°C
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:
FiguresAtomic Absorption
UV-Vis Images
|