User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/09: Difference between revisions
From OpenWetWare
(Autocreate 2014/04/09 Entry for User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab) |
(fix raw html notebook nav) |
||
(11 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objective== | ||
* | * Take conductivity measurements on 4°C solutions from 3/26/14. | ||
* Run UV-Vis and AA on solutions at 36°C from 3/26/14. | |||
==Procedure== | |||
===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C=== | |||
*Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated. | |||
*Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects. | |||
[[Image:4.9.conductivity.4.png|800px|]] | |||
===Atomic Absorption Preparation=== | |||
'''Creating the Gold Stock Solutions''' | |||
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio at 36°C were run on the AA: | |||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | |||
==Figures== | |||
===Atomic Absorption=== | |||
*In comparison to yesterday this AA data has a more recognizable trend. Overall it appears that the amount of gold in solution decreases as the concentration of variable increases. | |||
[[Image:4.9.aa.hot.png|700px|]] | |||
* 2,2 bipyridine data was just as wonky as yesterday by having an increase in gold followed by a prompt and steep decrease. | |||
[[Image:4.9.aa.hot.2.2.bipy.png|700px|]] | |||
==UV-Vis Images== | |||
*Please note that very few samples actually had suspended nanoparticles in solution. | |||
[[Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png]] | |||
[[Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png]] | |||
[[Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png]] | |||
[[Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png]] | |||
*Note that highlighted samples are the only samples with enough of a recognizable peak to calculate an equilibrium constant. Some 2,2 bipyridine data is being left out because it showed no variation from the control. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 23:53, 26 September 2017
Biomaterials Design Lab: Spring 2014 | Main project page Previous entry Next entry |
Objective
ProcedureConductivity Measurements of 30:1 Lysozyme-AuNP at 4°C
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:
FiguresAtomic Absorption
UV-Vis Images
|