User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/05: Difference between revisions

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===Figure 2. Atomic Absorbance of BSA with MgCl2 added before being heated===
===Figure 2. Atomic Absorbance of BSA with MgCl2 added before being heated===
 
*With the BSA samples baked with salt their doesn't appear to be any recognizable trend.
[[Image:BSA with MgCL2 Javier Vinals Camallonga.png]]
[[Image:BSA with MgCL2 Javier Vinals Camallonga.png]]


Revision as of 15:42, 17 April 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Calculate equilibrium constants for heated 30:1 lysozyme:AuNP (with variables added after baking) and room temperature 30:1 lysozyme:AuNP (with variables added after baking).
  • Make standard with amount of gold per 5 mL tube to run AA and UV-Vis.
  • Centrifuge samples from yesterday (30:1 lysozyme-AuNP with variables added after heating at room temperature).
  • Run AA and UV-Vis on the centrifuged samples.

Procedure

Atomic Absorption

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
  6. A sixth stock solution of gold was created with 448 μL of HAuCl4·3H2O and 4552 μL of distilled water to a Falcon tube. This resulting concentration represents the amount of gold in a 5 mL sample of lysozyme-AuNP.


Atomic Absorption Samples

The following samples were run on the AA:

  • 10 μg/mL Au, 20 μg/mL Au, 30 μg/mL Au, 40 μg/mL Au, 50 μg/mL Au
  • 30:1 lysozyme-AuNP with NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid
  • 60:1, 70:1, 80:1, 90:1, 100:1 BSA-AuNP with MgCl2
  • Gold solution in 5 mL sample of lysozyme-AuNP
  • Control of 30:1 lysozyme-AuNP without any added variable

Figures

Atomic absorbance of Lysozyme with different concentrations of variables

  • All of the data appears to follow the same trend. The amount of gold appears to decrease in solution as the concentration of variable increases.


Figure 2. Atomic Absorbance of BSA with MgCl2 added before being heated

  • With the BSA samples baked with salt their doesn't appear to be any recognizable trend.



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