User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/12

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(Procedure)
Current revision (17:38, 16 February 2014) (view source)
(Procedure)
 
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* 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1  110:1 ------- BSA: 100:1
* 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1  110:1 ------- BSA: 100:1
* All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.  
* All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.  
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* To the pure fiber samples chelators, and buffers were added.  
+
* To the pure fiber samples and BSA AuNP's chelators, and buffers were added.  
*At this point we ran out of time.   
*At this point we ran out of time.   

Current revision

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Take 1 mL aliquots of lysozyme-AuNP and BSA-AuNP synthesized yesterday.
  • Centrifuge aliquots and remove supernatant.
  • Retest all treatments with samples of pure fibers.

Procedure

  • 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1 110:1 ------- BSA: 100:1
  • All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.
  • To the pure fiber samples and BSA AuNP's chelators, and buffers were added.
  • At this point we ran out of time.


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