User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/12

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(Autocreate 2014/02/12 Entry for User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab)
Current revision (17:38, 16 February 2014) (view source)
(Procedure)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Objective==
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* Insert content here...
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* Take 1 mL aliquots of lysozyme-AuNP and BSA-AuNP synthesized yesterday.  
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* Centrifuge aliquots and remove supernatant.  
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* Retest all treatments with samples of pure fibers.
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==Procedure==
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* 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1  110:1 ------- BSA: 100:1
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* All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.
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* To the pure fiber samples and BSA AuNP's chelators, and buffers were added.
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*At this point we ran out of time. 
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Current revision

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Take 1 mL aliquots of lysozyme-AuNP and BSA-AuNP synthesized yesterday.
  • Centrifuge aliquots and remove supernatant.
  • Retest all treatments with samples of pure fibers.

Procedure

  • 1ml aliquots were prepared for all newly synthesized AuNP's. Lysozyme: 35:1 55:1 110:1 ------- BSA: 100:1
  • All 1ml tubes for the 55:1 and 110:1 ratios were spun down in a centrifuge. Supernatant was removed in order to get pure fibers.
  • To the pure fiber samples and BSA AuNP's chelators, and buffers were added.
  • At this point we ran out of time.


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